Salicylate Hydroxylase catalyses the conversion of salicylate and NADH to catechol and NAD+ in the presence of oxygen. The resulting decrease in absorbance at 340nm, due to the conversion of NADH to NAD+, is proportional to the concentration of Salicylate in the sample.
The intended use is for the quantitative measurement of salicylate in serum / plasma as an aid to monitor and diagnose salicylate overdose. Linearity is 0.05 – 10.00 mmol/L (6 – 1380 mg/L).
The kit is adaptable for use on multiple clinical chemistry analysers.
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